COMPARISON OF HEMATOXYLIN & EOSIN (H&E) STAINING AND PERIODIC ACID SCHIFF-ALCIAN BLUE (PAS-AB) HISTOCHEMISTRY IN ESOPHAGEAL BIOPSIES IN TERMS OF INTESTINAL METAPLASIA

Barret esophagus is considered as a precursor lesion for esophageal adenocarcinomas. Histopathological examination required the barret esophagus, where endoscopic takes an important place. Intestinal metaplasia revealed with hematoxylin & eosin (H&E) and alcian blue (AB) in histopathological examinations. There are 2 different opinions on this issue. One of them to perform histochemical AB or PAS-AB when intestinal suspected routine H&E staining, while other Periodic acid schiff (PAS-AB) all biopsies section. This study aims reveal roles staining methods assessment metaplasia. 200 were included study. Sections re-evaluated blindly by two pathologists. stains compared terms sensitivity, specificity, positive predictivity. In statistical analysis, strong correlation was found between evaluation (Kendall, p = 0.00; r 0, 81). evaluation, sensitivity 79%, specificity 100%, predictivity negative 82.6%, 100% stained sections. The main goal detect cases. Since absence means less importance, higher values should be preferred rather than observed Considering these conditions, it seems rational use auxiliary histochemistry containing AB.